RT - Journal TY - JOUR A1 - Kumar, Meenakshi A1 - Abraham, Ambily A1 - Savithri, Handanahal A1 - Karande, Anjali T1 - Nanocarrier mediated intracellular delivery of neutralizing antibodies for the management of abrin lethality YR - 2017/4/1 JF - Biomedical Research Journal JO - Biomed Res J SP - 67 OP - 81 VO - 4 IS - 1 UL - https://www.brjnmims.org/article.asp?issn=2349-3666;year=2017;volume=4;issue=1;spage=67;epage=81;aulast=Kumar;t=5 DO - 10.4103/2349-3666.240592 N2 - Abrin is a highly lethal ribosome-inactivating protein of high relevance in bio-warfare. Neutralizing antibodies to abrin reported so far afford protection in a very small window with no rescue observed post-abrin exposure. The utility of the most specific mode of defense against abrin poisoning is thus undermined. We proposed that intracellular delivery of antibodies which otherwise lack the ability to enter cells would increase the therapeutic potential. Towards this, we analyzed the use of silica nanoparticles and chimeric Sesbania mosaic virus-like particles in delivering antibodies inside cells. The antibody-nanocarrier conjugate was found to be effective in protecting cells from abrin-mediated toxicity when administered as long as 9 h prior and up to 1 h post abrin exposure. These results highlight the potential of nanocarrier-based intracellular delivery of neutralizing antibodies in widening the therapeutic window for prophylaxis and to some extent post-exposure treatment of abrin poisoning. ER -