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Figure 5: SPR analysis showing the real-time binding of cell surface protein fraction (CSP) of LNCaP cells containing estrogen receptors to immobilized polyclonal ERα and ERβ antibodies. Sensogram showing the binding of CSP (500ng-8μg), flowed over a surface of sensor chip immobilized with antibody specific against estrogen receptor alpha and estrogen receptor beta respectively (a and c). Sensograms showing the binding of CSP (1μg) to estrogen receptor alpha and estrogen receptor beta antibodies immobilized over a sensor chip in absence and presence of different concentrations of estradiol (1μM and 10μM) (b and d). |
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